Click here to close now.


News Feed Item

Avid Bioservices to Highlight Novel Methods for Increasing Protein Expression and Developing an Optimized Upstream Process at IBC's Biopharmaceutical Development & Production Week Conference

Studies Conducted With a Suspension CHO Expression System Resulted in Specific Feeds, Nutrient Concentration, and Optimized Process Parameters That Increased Titer by 10-Fold

TUSTIN, CA -- (Marketwired) -- 03/24/14 -- Avid Bioservices, Inc., a wholly owned subsidiary of Peregrine Pharmaceuticals, Inc., today announced that recent studies evaluating a new suspension Chinese Hamster Ovary (CHO) expression system identified several process parameters and a combination of feeds that significantly increased titer and productivity. The project included the selection of stable high producing subclones using ClonePix™ FL technology, optimizing media feeds through an intensive screening program and further characterization of the design space. The study resulted in a 10x improvement in productivity, while yielding a comparable product profile. The case study detailing these findings will be presented at IBC's Biopharmaceutical Development & Production Week Conference, to be held in San Diego, CA, March 24-27, 2014.

"The goal of this project was to implement a new expression technology and to apply our expertise in upstream process development to achieve a high producing cell line for the client. The result was a project that included screening of seventeen chemically defined, animal component free media and feeds and evaluating their effect on titer. In addition, we applied Design of Experiments (DoE) methodologies to map out the design space and eventual verification at larger scale. The result was an approximate 10-fold increase in titer with a comparable product," said Dr. Jeanette Doerr, Senior Scientist of Process Sciences at Avid Bioservices and leader of the project. "When our team applied these techniques to additional molecules, the results were consistent with the initial study, demonstrating that we can apply this approach to our clients' molecules with a high level of confidence in achieving similar successes. With a 10-fold increase in titer overall, we are optimistic that the upstream process developed during these studies has the potential to become a platform process."

As part of its integrated services portfolio from plasmid to delivery of drug substance or drug product, Avid offers cell line development and cell culture optimization services to clients under GMP, including, transfection, subcloning, clone selection using ClonePix technology and generation of R&D material to support early downstream development and pre-clinical studies. Following delivery of an optimized cell line, a robust upstream process is developed through the use of tools such as DoE and Quality by Design (QbD) studies, and optimized and characterized through media/feed screening that utilizes high throughput (HTPD) to increase titer, while maintaining critical quality attributes (CQAs).

"We have been working steadily to implement new technologies at Avid that will help our clients as they move their products toward commercialization. This case study is a good example of our ability to implement new technologies and to apply our core expertise in process optimization and product characterization in order to meet our customers' needs," said Connie Chang, Director of Process Sciences at Avid Bioservices. "Our goal at Avid is to be a partner for product development throughout the clinical trial process, helping clients to meet tight development timelines, while implementing consistent improvements that will allow them to advance to later stage clinical development and to eventually commercialize their products. This new expression technology appears to be robust and gives us another great option to offer to our clients."

Poster Presentation Details:

Title: "Cell Line Development and Upstream Process Optimization of a Suspension CHO Expression System"
Dates: Monday, March 24, 2014 -Tuesday, March 25, 2014
Authors: Jeanette Doerr, Ryan Rosson, Calla Montelongo, Vince Nguyen, Connie Chang

In addition, Avid Bioservices will be hosting conference attendees at booth #304 in the exhibit hall Monday, March 24 - Thursday, March 27, 2014.

For additional information on IBC's Biopharmaceutical Development & Production Conference, please visit:

About Avid Bioservices
Avid Bioservices provides a comprehensive range of high quality cGMP manufacturing services for the biotechnology and biopharmaceutical industries to support the manufacture of cGMP clinical phase and commercial products. With over 15 years of experience producing monoclonal antibodies and recombinant proteins in batch, fed-batch and perfusion modes, Avid's services include GMP clinical and commercial product manufacturing, purification, bulk packaging, final product filling, stability testing and regulatory strategy, submission and support. The company also provides a variety of process development activities, including cell line optimization, cell culture and feed optimization, analytical methods development and product characterization. For more information about Avid, please visit

About Peregrine Pharmaceuticals
Peregrine Pharmaceuticals, Inc. is a biopharmaceutical company with a portfolio of innovative monoclonal antibodies in clinical trials focused on the treatment and diagnosis of cancer. The company is pursuing multiple clinical programs in cancer with its lead immunotherapy candidate bavituximab while seeking a partner to further advance its novel brain cancer agent Cotara®. Peregrine also has in-house cGMP manufacturing capabilities through its wholly-owned subsidiary Avid Bioservices, Inc. (, which provides development and biomanufacturing services for both Peregrine and third-party customers. Additional information about Peregrine can be found at

Add to Digg Bookmark with Add to Newsvine

Chris Keenan or Kelly Pisarev Lord
(800) 987-8256

More Stories By Marketwired .

Copyright © 2009 Marketwired. All rights reserved. All the news releases provided by Marketwired are copyrighted. Any forms of copying other than an individual user's personal reference without express written permission is prohibited. Further distribution of these materials is strictly forbidden, including but not limited to, posting, emailing, faxing, archiving in a public database, redistributing via a computer network or in a printed form.

Latest Stories
DevOps is here to stay because it works. Most businesses using this methodology are already realizing a wide range of real, measurable benefits as a result of implementing DevOps, including the breakdown of inter-departmental silos, faster delivery of new features and more stable operating environments. To take advantage of the cloud’s improved speed and flexibility, development and operations teams need to work together more closely and productively. In his session at DevOps Summit, Prashanth...
DevOps has often been described in terms of CAMS: Culture, Automation, Measuring, Sharing. While we’ve seen a lot of focus on the “A” and even on the “M”, there are very few examples of why the “C" is equally important in the DevOps equation. In her session at @DevOps Summit, Lori MacVittie, of F5 Networks, will explore HTTP/1 and HTTP/2 along with Microservices to illustrate why a collaborative culture between Dev, Ops, and the Network is critical to ensuring success.
Containers have changed the mind of IT in DevOps. They enable developers to work with dev, test, stage and production environments identically. Containers provide the right abstraction for microservices and many cloud platforms have integrated them into deployment pipelines. DevOps and containers together help companies achieve their business goals faster and more effectively. In his session at DevOps Summit, Ruslan Synytsky, CEO and Co-founder of Jelastic, will review the current landscape of...
Today air travel is a minefield of delays, hassles and customer disappointment. Airlines struggle to revitalize the experience. GE and M2Mi will demonstrate practical examples of how IoT solutions are helping airlines bring back personalization, reduce trip time and improve reliability. In their session at @ThingsExpo, Shyam Varan Nath, Principal Architect with GE, and Dr. Sarah Cooper, M2Mi's VP Business Development and Engineering, will explore the IoT cloud-based platform technologies driv...
In their session at DevOps Summit, Asaf Yigal, co-founder and the VP of Product at, and Tomer Levy, co-founder and CEO of, will explore the entire process that they have undergone – through research, benchmarking, implementation, optimization, and customer success – in developing a processing engine that can handle petabytes of data. They will also discuss the requirements of such an engine in terms of scalability, resilience, security, and availability along with how the archi...
DevOps is gaining traction in the federal government – and for good reasons. Heightened user expectations are pushing IT organizations to accelerate application development and support more innovation. At the same time, budgetary constraints require that agencies find ways to decrease the cost of developing, maintaining, and running applications. IT now faces a daunting task: do more and react faster than ever before – all with fewer resources.
Overgrown applications have given way to modular applications, driven by the need to break larger problems into smaller problems. Similarly large monolithic development processes have been forced to be broken into smaller agile development cycles. Looking at trends in software development, microservices architectures meet the same demands. Additional benefits of microservices architectures are compartmentalization and a limited impact of service failure versus a complete software malfunction....
As a company adopts a DevOps approach to software development, what are key things that both the Dev and Ops side of the business must keep in mind to ensure effective continuous delivery? In his session at DevOps Summit, Mark Hydar, Head of DevOps, Ericsson TV Platforms, will share best practices and provide helpful tips for Ops teams to adopt an open line of communication with the development side of the house to ensure success between the two sides.
Docker is hot. However, as Docker container use spreads into more mature production pipelines, there can be issues about control of Docker images to ensure they are production-ready. Is a promotion-based model appropriate to control and track the flow of Docker images from development to production? In his session at DevOps Summit, Fred Simon, Co-founder and Chief Architect of JFrog, will demonstrate how to implement a promotion model for Docker images using a binary repository, and then show h...
The last decade was about virtual machines, but the next one is about containers. Containers enable a service to run on any host at any time. Traditional tools are starting to show cracks because they were not designed for this level of application portability. Now is the time to look at new ways to deploy and manage applications at scale. In his session at @DevOpsSummit, Brian “Redbeard” Harrington, a principal architect at CoreOS, will examine how CoreOS helps teams run in production. Attende...
There will be 20 billion IoT devices connected to the Internet soon. What if we could control these devices with our voice, mind, or gestures? What if we could teach these devices how to talk to each other? What if these devices could learn how to interact with us (and each other) to make our lives better? What if Jarvis was real? How can I gain these super powers? In his session at 17th Cloud Expo, Chris Matthieu, co-founder and CTO of Octoblu, will show you!
Developing software for the Internet of Things (IoT) comes with its own set of challenges. Security, privacy, and unified standards are a few key issues. In addition, each IoT product is comprised of at least three separate application components: the software embedded in the device, the backend big-data service, and the mobile application for the end user's controls. Each component is developed by a different team, using different technologies and practices, and deployed to a different stack/...
Clutch is now a Docker Authorized Consulting Partner, having completed Docker's certification course on the "Docker Accelerator for CI Engagements." More info about Clutch's success implementing Docker can be found here. Docker is an open platform for developers and system administrators to build, ship and run distributed applications. With Docker, IT organizations shrink application delivery from months to minutes, frictionlessly move workloads between data centers and the cloud and achieve 2...
Mobile messaging has been a popular communication channel for more than 20 years. Finnish engineer Matti Makkonen invented the idea for SMS (Short Message Service) in 1984, making his vision a reality on December 3, 1992 by sending the first message ("Happy Christmas") from a PC to a cell phone. Since then, the technology has evolved immensely, from both a technology standpoint, and in our everyday uses for it. Originally used for person-to-person (P2P) communication, i.e., Sally sends a text...
SYS-CON Events announced today that Sandy Carter, IBM General Manager Cloud Ecosystem and Developers, and a Social Business Evangelist, will keynote at the 17th International Cloud Expo®, which will take place on November 3–5, 2015, at the Santa Clara Convention Center in Santa Clara, CA.